నైరూప్య

Immunological characterization of flying and flightless birds based on Riboflavin binding protein (RfBP)

G.Rajender, G.Benarjee, M.S.K.Prasad, B.Laxma Reddy

Flying bird Peacock (Pavo cristatus), Hen (Gallus gallus) and flightless bird Emu (Dromius) egg Riboflavin binding protein (RfBP) was isolated from these birds. The Rfbp was purified in two steps, DEAE-Sephadex A-50 ion exchange chromatography. The final purification of proteins (Rfbp) was achieved on Sephadex G-100. Emu Rfbp contains more non specific protein and contaminants. To avoid this non specific protein column purification repeatedly column filteration.The protein content was estimated with Lowry method. The purity of the proteins was judged by cylindrical and slab–gels, Electrophoresis techniques. The final purified proteins showed a single band on electrophoresis gels and the molecular weight was 30 Kilodaltons. Antiserum was raised against these Rfbp’s in rabbit. This antiserum showed Immunological cross reactivity (precipitation line) between the two compounds in flying birds. In flightless birds there was no cross reactivity. This study proved that, these two birds (flying and flightless birds) are immunologically relative and phylogentically different from each other.