నైరూప్య

Determination of mycophenolate mofetil in bulk and pharmaceutical formulations by UV derivative spectrophotometric method

T.Vijaya Bhaskara Reddy, N.Sowjanya Reddy, G.Ramu, S.Ravi Prakash Reddy, C.Rambabu

Simple and rapid UV absorption spectrophotometric (Method-A) and UV second order derivative spectrophotometric (Method-B) methods have been developed for the determination ofMycophenolateMofetil (MMF) in pure and its pharmaceutical formulations. The absorption spectrum, first and second order derivative spectra of working standard solution of concentration 20µg/mL of MMF in methanol was recorded against methanol as a reagent blank and wavelength of maximum absorbance was found to be 249.0nm. From the first derivative spectrum it was found that a valley at 257.4nmshowed maximumamplitude. Second derivative spectrumhas the maximumamplitude in negative valley at 249.0nmhence the second derivative method was validated bymeasuring amplititudes at 249.0nm. Standard deviation and percent of relative standard deviation of six replicates were calculated and found to be within the limits. The mean percent of recovery were evaluated at 50%, 100% and 150% concentration levels and found to be within the range 99.1-99.6 percent. The developed methods were found to be linear within the range of concentrations 10-30ìg/mL. Slope, intercept and correlation coefficient for the developed method were calculated and found to be satisfactory. The methods have been proved robust and rugged. The developed methods were found to be precise, accurate and stable, therefore readily adapted for routine quality control of MMF by ordinary laboratories. The developed methods were effective for quantitative determination of MMF in bulk and pharmaceutical preparations without any interference of other constitute in tablets of different brand names.